Skip to main content


You are viewing the new BMC article page. Let us know what you think. Return to old version

Research Article | Open | Published:

Evaluation of Robust Estimators Applied to Fluorescence Assays


We evaluated standard robust methods in the estimation of fluorescence signal in novel assays used for determining the biomolecule concentrations. The objective was to obtain an accurate and reliable estimate using as few observations as possible by decreasing the influence of outliers. We assumed the true signals to have Gaussian distribution, while no assumptions about the outliers were made. The experimental results showed that arithmetic mean performs poorly even with the modest deviations. Further, the robust methods, especially the -estimators, performed extremely well. The results proved that the use of robust methods is advantageous in the estimation problems where noise and deviations are significant, such as in biological and medical applications.

Publisher note

To access the full article, please see PDF.

Author information

Correspondence to M. Västilä.

Rights and permissions

Reprints and Permissions

About this article


  • Information Technology
  • Quantum Information
  • Full Article
  • Fluorescence Assay
  • Robust Estimator